Figure 4.—

Hri2p is the predominant eIF2 kinase responding to arsenite stress. Five isogenic strains expressing the entire collection of eIF2 kinases (WT), the indicated eIF2 kinases, or devoid of all three eIF2 kinases (none) were incubated with sodium arsenite for 1, 3, or 6 hr or no stress (0 hr). Cells were collected and washed and equal amounts of lysate based on protein content were analyzed by immunoblot using antibody that specifically recognizes phosphorylated eIF2α (eIF2α-P) or antiserum that recognizes total eIF2 (eIF2α).