Figure 5.—

Both Δyar1 and Δltv1 strains are hypersensitive to protein synthesis inhibitors. The sensitivity of wild-type (WT) and isogenic Δyar1 and Δltv1 cells to various protein synthesis inhibitors was tested by growing cells as described in Figure 1. (A) A total of 16,000, 4000, or 1000 cells (left to right) were spotted onto YPD plates with or without the denoted drugs. Only the 1000-cell column is shown for the neomycin (neo; 1 mg/ml) and paromomycin (paro; 1 mg/ml) plates. (B) A total of 400 cells of each strain were spotted onto YPD, YPD + cycloheximide (CHX; 1μg/ml), or YPD + anisomycin (Anis; 7.5 μg/ml) plates. Equal numbers of wild-type and mutant cells were spotted at each dilution. Plates were incubated at 30° unless otherwise indicated.