TABLE 3.
Homologous and homeologous recombination rates insgs1 mutants
| Homologous recombination
|
Homeologous recombination
|
|||
|---|---|---|---|---|
| Relevant genotype | Lys+ rate × 10−8 | Relative rate |
His+ rate × 10−8 | Relative rate |
| Wild type | 230 (210–270) | 1.0 | 4.2a (4.0–4.7) | 1.0 |
| sgs1Δ | 2500 (2100–2700) | 11 | 300 (270–310) | 71 |
| sgs1Δ + vector | 1900 (1300–2500) | 8.1 | 170 (130–260) | 41 |
| sgs1Δ + SGS1 | 190 (150–230) | 0.84 | 3.0 (2.4–3.5) | 0.71 |
| sgs1Δ + sgs1-hd | 1500 (1300–1900) | 6.6 | 130 (110–170) | 31 |
| sgs1Δ + sgs1-ΔN158 | 2700 (1400–3400) | 12 | 230 (140–260) | 55 |
| sgs1Δ + sgs1-ΔN158hd | 1900 (1600–2600) | 8.4 | 190 (130–220) | 46 |
| sgs1Δ + sgs1-ΔN322 | 2200 (1600–2800) | 9.7 | 130 (64–260) | 32 |
| sgs1Δ + sgs1-ΔN644 | 1100 (910–1500) | 4.7 | 50 (36–68) | 12 |
| sgs1Δ + sgs1-ΔC200 | 170 (130–190) | 0.72 | 13 (8.5–18) | 3.2 |
| sgs1Δ + sgs1-ΔC300 | 1300 (950–1800) | 5.8 | 75 (61–84) | 18 |
| sgs1Δ + sgs1-ΔC795 | 1000 (870–1100) | 4.3 | 91 (76–110) | 22 |
Homologous recombination (Lys+) and homeologous recombination (His+) in null sgs1Δ::kan mutants (sgs1Δ) containing a wild-type or mutant version of SGS1 integrated at the ADE2 locus were compared to that of the wild-type SGS1 parent. The mutant alleles of SGS1 (full-length 1447 amino acids) contain the helicase-defective allele (K706A) and/or terminal deletions of the indicated number of amino acids from the amino (N) or the carboxy (C) terminus (Mullen et al. 2000). Confidence intervals of 95% are indicated within parentheses. The relative rate was calculated by dividing the mutant rate by the wild-type rate.
a
Rate is from Spell and Jinks-Robertson (2003).