Figure 3.—

Diurnal expression patterns of per and cry mRNA in whole head of male flies. Similar results were obtained for female flies. The transcription levels of Bactrocera per and cry in the whole head of B. tryoni and B. neohumeralis were measured in 4-hourly cDNA samples from flies maintained in 24-hr light-dark cycles. The relative abundance of per and cry mRNA is shown as a percentage of kanamycin mRNA on a log scale. cry mRNA is the mean of three independent real-time PCR assays. Open, solid, and hatched bars are the hours of lights on, lights off, and dusk lighting, respectively. Solid line, B. neohumeralis; dashed line, B. tryoni.