Figure 5.—

Mutation and overexpression of Lk6 leads to opposite effects on the aret phenotype. Ovaries from aret^PD/aret^QB females arrest oogenesis at stage 6 or 7 (A), but the arrest is delayed when the females are also heterozygous for Df(3R)M-Kx-1/+ (B). A single copy of the EP(3)0886 P-element insertion in Lk6 has a similar effect (C). Conversely, overexpression of Lk6 using EP(3)0886 and a germline GAL4 driver (matα4-GAL4-VP16#6) enhances the aret phenotype (D). The presence of the GAL4 driver alone does not change the aret phenotype (E). In all cases the aret combination was aret^PD/aret^QB, and the ovaries were stained with ToPro-3.