. 2004 Nov;168(3):1763–1771. doi: 10.1534/genetics.104.029033

TABLE 1.

Synthetic interaction results

ORF	Gene name	Function^a	SI with pds1Δ^b	SI with mad2^c^,^d	SI with rad9Δ^c^,^e	SI with mrc1Δ^c^,^f	Rescue of SI with pds1Δ by 2μESP1^g
Microtubule dynamics
YMR198W	CIK1	Kar3 kinesin-binding protein	Lethal	Lethal	None	Sick	No
YOR349W	CIN1	Tubulin-folding chaperone	Lethal	Lethal	None	None	No
YPL241C	CIN2	Tubulin-folding chaperone	Lethal	None	None	None	No
YEL061C	CIN8	Spindle-associated kinesin	Lethal	Lethal	None	None	No
YML094W	GIM5	Tubulin folding	Lethal	Sick	None	None	No
Chromosome segregation
YPL008W	CHL1	Helicase, sister chromatid cohesion	Sick	None	None	None	No
YPR135W	CTF4	Associated with DNA polα	Sick	None	None	Lethal	No
YRM078C	CTF18	Alternative RF-C subunit	Lethal	None	None	None	No
YPL018W	CTF19	Kinetochore protein	Lethal	Lethal	None	None	No
YCL016C	DCC1	Alternative RF-C subunit	Lethal	None	None	None	No
YOR195W	SLK19	Kinetochore and spindle associated	Lethal	None	None	None	No
Cell cycle
YPR119W	CLB2	Mitotic cyclin	Sick	None	None	None	No
YCR065W	HCM1	Transcription factor	Sick	Sick	None	None	No
Heat Shock
YMR186W	HSC82	Hsp90-type chaperone/heat shock	Sick	None	None	None	Yes
YOR027W	STI1	Hsp90 cochaperone	Sick	None	None	None	Yes
YPL106C	SSE1	Hsp70-type chaperone/heat shock	Lethal	None	None	None	No
Unknown function
YOR080W	DIA2	F-box protein	Lethal	None	Lethal	None	No
YLR204W	QRI5	Unknown	Sick	None	None	None	No
YNL081C	SWS2	Unknown	Sick	None	None	None	No
YIL040W	APQ12	Unknown	Lethal	Lethal	None	None	No
YDR200C	VPS64	Mating-pheromone response	Sick	Lethal	None	None	No

SI, synthetic interaction.

^{^a}

Function is based on the gene ontology annotation of the Saccharomyces cerevisiae database (http://www.yeastgenome.org/) and references therein.

^{^b}

pds1Δ strain used was described in legend to Figure 2.

^{^c}

Crosses to mad2Δ, rad9Δ, and mrc1Δ strains were done with single mutants (gene_nΔ) that came out of the tetrad analysis done in the cross with pds1Δ.

^{^d}

Deletion of the MAD2 gene results in the inactivation of the spindle checkpoint pathway. The mad2Δ strain used was MATα can1Δ leu2Δ his3Δ lys2Δ ura3Δ mfa1Δ::MFApr-HIS3 mad2::NAT[pRL18] (pRL18: CEN URA3 MAD2 plasmid). Strain and plasmid were a gift from Dan Burke (University of Virginia).

^{^e}

Deletion of the RAD9 gene results in the inactivation of the DNA damage checkpoint pathway. The rad9Δ strain used was MATa ura3Δ leu2Δ his3Δ met15Δ rad9::NAT [pRAD9] (pRAD9: CEN URA3 RAD9 plasmid). pRAD9 was a gift from Steve Elledge (Harvard Medical School).

^{^f}

Deletion of the MRC1 gene results in the inactivation of the DNA replication checkpoint pathway. The mrc1Δ strain used was MATa ura3Δ leu2Δ his3Δ met15Δ mrc1::NAT [pMRC1] (pMRC1: CEN URA3 MRC1 plasmid). pMRC1 was a gift from Steve Elledge (Harvard Medical School).

^{^g}

Plasmid 2μESP1 is a pRS426 (2μURA3)-based plasmid encoding for ESP1 in which the URA3 gene was substituted for LYS2 by PCR-mediated recombination. This plasmid was transformed into pds1Δ gene_nΔ lys2 strains carrying pPDS1 that were isolated in the original tetrad analysis.