Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The targeting scheme. (A) Regions flanking the locus to be deleted, indicated as L and R, were cloned into the P-element vector, pW25, with the indicated features. FLP and I-SceI generate the extrachromosomal linear donor, as shown. (B) Recombination of the Hsp70A, Hsp70Ba, and Hsp70B donors should generate the indicated deletions. The approximate locations of SphI (Sp) sites used in subsequent analyses are indicated.
