Figure 4.—

Combined agroinfiltration of Avr9 under control of the 35S promoter and Hcr9's under control of the 35S or their native promoter. (A) Agroinfiltration of 35S-driven constructs containing the 9DC genes, Cf-9 or 9D. 9DC1/2 represents the identical 9DC1 and 9DC2 coding regions. (B) Agroinfiltration assay of constructs containing 9DC1, 9DC2, or 9DC3 under control of their native promoter; 35S-driven Cf-9 was used as a control. Expression of an Hcr9 that confers Avr9 responsiveness results in visible necrosis. Both photographs were taken 7 days after infiltration.