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. 2004 Nov;168(3):1539–1555. doi: 10.1534/genetics.104.029215

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Copyright © 2004, Genetics Society of America

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Figure 7.— — DNA sequence analysis of mutated Cμ-region positions. Parental DNA consists of the wild-type chromosomal sequence containing the MfeI site and of the corresponding vector-borne sequence in which the MfeI site is replaced by the 30-bp palindrome containing the diagnostic NotI site. The observed sequences spanning the site corresponding to the MfeI/NotI mismatch in the 5′ Cμ region of recombinants 10-2 and 9-1 and the 3′ Cμ region of recombinants 89-2 and 28-1 are indicated. The deletion endpoints that correspond to the observed sequences are also presented.