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. 2004 Nov;168(3):1539–1555. doi: 10.1534/genetics.104.029215

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Copyright © 2004, Genetics Society of America

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Figure 8.— — Variations of the canonical DSBR model. (A) The initial strand invasion event produces a very short region of heteroduplex on one side of the DSB, following which primed DNA synthesis generates a much more extensive length of heteroduplex involving the noninvading strand on the other side of the DSB. (B) The initial strand invasion event proceeds according to the canonical DSBR model. However, branch migration in the direction of the newly synthesized DNA generates an intermediate in which the two Holliday junctions and the intervening hDNA are located on the same side of the DSB. In both A and B, alternate sense cleavage generates the crossover product. Initially, the marker segregation pattern is 4:4, 5:3 on the two sides of the DSB. However, the 5:3 marker may be altered through repair to yield a 4:4 or a 6:2 segregation. (C) As in the canonical DSBR model, heteroduplexes form on each side of the DSB, producing an intermediate with two HC events (5:3; 5:3 segregation). Repair activity directed by cutting of the two Holliday junctions in the favored sense (Gilbertson and Stahl 1996; Foss et al. 1999; Baker and Birmingham 2001) can result in restoration and/or conversion events. A repair event issuing from cut junction A results in restoration of normal 4:4 segregation (pathway 1), conversion toward the chromosomal sequence as shown for repair events directed by cut junction B (pathway 2), or both restoration and conversion as shown for repair events directed by cut junctions A and B (pathway 3). Restoration-conversion products also result from repair events directed by junction cuts at C and/or D, and for those involving various combinations of junction cuts at A, B, C, or D, other outcomes are possible (not illustrated).

Figure 8.— — Variations of the canonical DSBR model. (A) The initial strand invasion event produces a very short region of heteroduplex on one side of the DSB, following which primed DNA synthesis generates a much more extensive length of heteroduplex involving the noninvading strand on the other side of the DSB. (B) The initial strand invasion event proceeds according to the canonical DSBR model. However, branch migration in the direction of the newly synthesized DNA generates an intermediate in which the two Holliday junctions and the intervening hDNA are located on the same side of the DSB. In both A and B, alternate sense cleavage generates the crossover product. Initially, the marker segregation pattern is 4:4, 5:3 on the two sides of the DSB. However, the 5:3 marker may be altered through repair to yield a 4:4 or a 6:2 segregation. (C) As in the canonical DSBR model, heteroduplexes form on each side of the DSB, producing an intermediate with two HC events (5:3; 5:3 segregation). Repair activity directed by cutting of the two Holliday junctions in the favored sense (Gilbertson and Stahl 1996; Foss et al. 1999; Baker and Birmingham 2001) can result in restoration and/or conversion events. A repair event issuing from cut junction A results in restoration of normal 4:4 segregation (pathway 1), conversion toward the chromosomal sequence as shown for repair events directed by cut junction B (pathway 2), or both restoration and conversion as shown for repair events directed by cut junctions A and B (pathway 3). Restoration-conversion products also result from repair events directed by junction cuts at C and/or D, and for those involving various combinations of junction cuts at A, B, C, or D, other outcomes are possible (not illustrated).