Figure 6.—

Western blots of total protein extracts from S. coprophila, R. Americana, and T. pubescens. The anti-Sxl against the S. ocellaris Sxl protein was used (Ruiz et al. 2003). The preimmune serum does not give any signal in Western blots (data not shown). For S. coprophila: lane 1, female post-blastoderm embryos; lane 2, male post-blastoderm embryos; lane 3, salivary glands of female larvae, lane 4, salivary glands of male larvae; lane 5, head an thorax of adult females; and lane 6, head and thorax of adult males. The identification of sexual phenotype of embryos and larvae of S. coprophila is straightforward because this species is composed of female-producer (gynogenic) and male-producer (androgenic) females. In addition, the different size of the male and female gonads can be also used for sexing the larvae. For R. americana: lane 1, abdomen of adult females; lane 2, abdomen of adult males; lane 3, head and thorax of adult females; and lane 4, head and thorax of adult males. For T. pubescens: lane 1, abdomen of adult females; lane 2, abdomen of adult males; lane 3, head an thorax of adult females; and lane 4, head and thorax of adult males.