TABLE 1.
Summary information on the six SSRs used to identify LGs IV, V, and XI including length in base pairs and optimum annealing temperature
| SSR | Repeat | LG | Sequence | Length (bp) | Annealing temperature |
|---|---|---|---|---|---|
| STM5140 | (AAT)7 | IV | F: GCTATTGTTGCAGATAATACG | 180–190 | 45° |
| (estp104b1) | R: GCCATGCACTAATCTTTGA | ||||
| STM3160 | (AG)25 | IV | F: ACTCAGAACACCGAATGG | 105–127 | 54° |
| R: CTCCACTACTGGTCAAATCC | |||||
| STM3179 | (AT)10 | V | F: ACCATAACCATCATCGTCTAC | 168–185 | 54° |
| R: GATAGTGATTGGTGGTGATTC | |||||
| STM5148 | (GAA)17 | V | F: TCTTCTTGATGACAGCTTCG | 380–440 | 55° |
| R: ACCTCAGATAGTTGCCATGTCA | |||||
| STM5109 | (TA)9 | XI | F: ACAGCTTTACTCTGCTCAACAA | 208–212 | 57° |
| (estp2OB8) | R: TGACGGCGTTATTACAAAC | ||||
| STM5130 | (CGG)5 | XI | F: AAAGTACAGCGAAGATGACGAC | 240–250 | 57° |
| (estp21B15) | R: TTACCTTTGCAACCTTGCC |