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. 2004 Oct;168(2):855–865. doi: 10.1534/genetics.103.025965

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Copyright © 2004, Genetics Society of America

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Figure 8.— — The sensitivity of the ey-GAL4/UAS-D-DDB1(650)-dsRNA transgenic flies phenotype was enhanced by UAS-P35. (A) wild-type fly (+/+); (B) UAS-D-DDB1(650)-dsRNA/+; (C) ey-GAL4/UAS-P35; (D) DDB1^EY01408/+; (E) ey-GAL4,UAS-D-DDB1(650)-dsRNA/+; (F) ey-GAL4,UAS-D-DDB1(650)-dsRNA/+;DDB1^EY01408/+; (G) ey-GAL4,UAS-D-DDB1(650)-dsRNA/ey-GAL4,UAS-D-DDB1(650)-dsRNA; (H) ey-GAL4,UAS-D-DDB1(650)-dsRNA/UAS-P35. The P35, anti-apoptotic protein strongly enhances the the D-DDB1 gene silencing phenotype. Note that the fly carrying two copies of ey-GAL4 and two copies of UAS-D-DDB1(650)-dsRNA showed no head and died at the pupa stage. (G) The content of the dissected pupa.