Figure 7.—

Disruption of pcsA. (A) Schematic of replacement of pcsA with pyrG through a double-crossover event by transformation with the vector pYQ8.4. (B) Southern blotting revealed that pcsA was replaced by pyrG in transformant TYQ4.24 (lane 3) and two other transformants (lanes 7 and 10). Genomic DNA of the transformants and the wild type was digested with SacII and hybridized with the fragment released from pYQ8.4 (including the 5′ and 3′ flanking region of pcsA and pyrG gene, shown in A) as the probe. WT, wild-type DNA.