TABLE 3.
Properties of multicopy suppressors ofswi6-ts
| Yeast strain | FBA1 | RPL40a/ UBI1 | GIN4 | PAB1 |
|---|---|---|---|---|
| bck2Δ swi6-4 | + | + | + | + |
| bck2Δ swi6Δ GAL1:CLN2a | + | − | + | (above 30°) |
| bck2Δ cln3Δ GAL:CLN2a | − | + | − | + |
| mbp1Δ swi4Δ GAL:CLN2a | + | − | − | − |
| bck2Δ swi6-4 cln1Δ | + | + | + | + |
| bck2Δ swi6-4 cln2Δ | − | − | − | − |
Suppression (+) of the double deletion strains by the designated multicopy plasmid indicates a capacity of those cells to form colonies on YEPD plates at 30° unless otherwise indicated. Suppression (+) of the strains carrying the temperature-sensitive swi6-4 allele indicates a capacity to form colonies on YEPD plates at 32°.
a
These strains carry GAL-CLN2 integrated at the HIS2 locus. CLN2 at its genomic locus is wild type.