TABLE 3.
Gene |
RPL32 abundance as predictor (r2)a |
Range of residuals (CTs) |
≈ Transcript fold differenceb |
d.f. | F-ratioc |
---|---|---|---|---|---|
Acp26Aa | 0.82*** | −2.9–2.3 | 37d | 96,242 | 5.3*** |
CG8137 | 0.71*** | −1.7–2.4 | 13.3 | 95,227 | 6.5*** |
Acp29AB | 0.67*** | −1.0–1.5 | 5.2 | 94,187 | 3.4*** |
CG31872 | 0.80*** | −1.6–1.5 | 7.9 | 96,243 | 8.9*** |
Acp32CD | 0.73*** | −1.5–1.3 | 4.4 | 96,263 | 7.4*** |
Acp33A | 0.79*** | −2.0–1.5 | 10.7 | 96,237 | 5.3*** |
CG17331 | 0.85*** | −1.1–1.0 | 2.8 | 96,251 | 2.5*** |
Acp36DE | 0.76*** | −2.1–1.2 | 6.5 | 96,249 | 7.4*** |
Acp53Ea | 0.83*** | −1.3–1.5 | 7.0 | 96,254 | 4.9*** |
PEBII | 0.57*** | −5.9–2.8 | 349e | 96,262 | 2.8*** |
*P < 0.05, **P < 0.01, ***P ≤ 0.001 based on simple linear regression (see text).
Transcript fold difference calculated as: difference = (1 + PCR efficiency)(largest residual CT−smallest residual CT).
*P < 0.05, **P < 0.01, ***P ≤ 0.001 based on ANOVA test for heterogeneity among lines (see text).
Excluding outlier results in a 14-fold difference.
Excluding outlier results in a 33-fold difference. This outlier was not the same line as the outlier in Acp26Aa.