TABLE 5.

Genotype-to-phenotype associations for sperm competitive ability ^****

Phenotype	Marker	Frequency of “fit” allele	Marker typea	Adjusted r2b	Other associationsc
Defense
P1′	CG8137s1910**	0.84	Intron	0.08	33,17331 (+)
Acp33s2125**	0.78	Downstream	0.08	P2, Rem (+)
Fecundity-D	None	NA	NA	NA	NA
Refractoriness	CG17331s1421**	0.18	Upstream	0.06	P2 (−): FecO (+)
Offense
P2′	Acp26s2201****	0.72	AGC(S):ATA(I)	0.13	53 (−)
Acp29s2072**	0.73	ACA(un.):ACC(pref.)	0.07	P1 (+)
Acp33s2125****	0.78	Downstream	0.16	P1, Rem (+)
CG17331s1411**	0.91	Upstream	0.08	FecO (+)
CG17331s1421**	0.82	Upstream	0.08	Refr (−): FecO (+)
Fecundity-O	None	NA	NA	NA	NA
Remating	CG31872s4328**	0.34	CTT(un.):CTC(un.)	0.05	None

In defense tests the experimental male was the first male to mate. P1′ is the proportion of offspring sired by the first male. Fecundity-D is the total fecundity of doubly mated females in the defense tests. Refractoriness (Refr) is the proportion of females that did not remate after mating to experimental males. In offense tests the experimental male was the second male to mate. P2′ is the proportion of offspring sired by the second male. Fecundity-O (FecO) is the total fecundity of doubly mated females in the offense tests. Remating (Rem) is the proportion of females that remated in the offense tests.

^*Comparisonwise P < 0.05,

^**P < 0.01,

^***P ≤ 0.001,

^****Experimentwise P < 0.05. NA, not applicable.

^aHigh-fitness codon shown on the left with its amino acid for nonsynonymous changes or usage; preferred (pref.) and unpreferred (un.) for synonymous changes indicated in parentheses.

^bCalculated using a simple linear regression considering only the individually significant marker (see text).

^c“Other associations” indicates other phenotypes that were associated with that marker. Significant associations are underlined. Antagonistic associations indicated by (−) and protagonistic associations indicated by (+). Markers associated with both phenotype and expression were considered protagonistic if the same allele produced higher fitness and higher transcript abundance. Genes are listed excluding the “CG” or the “Acp” prefix.