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(A) The steps and results of the enrichment method approach. (B) Phenotypic comparison of the new cut9-41 allele and cut9-665. Strains cut9-41 (FY1304) and cut9-665 (FY1305) were grown at 25° to OD595 = 0.4 and shifted to 36°. Samples were collected every 2 hr and fixed for DAPI/calcoflour. Arrowheads indicate cells with chromosome and/or septation defects. Bar, 10 μm.
