Figure 2.—
(A) Scheme showing the steps and results of the screening method approach. (B) Example of interesting rereplication mutant isolated with the screening approach. Strains FY875 and FY3033 (rereplication candidate) were arrested in G1 by nitrogen starvation. Cultures were divided in two and released by adding nitrogen and thiamine. One culture was placed at 25° and the other at 36°. Samples were collected after 5 and 10 hr, ethanol fixed, and analyzed by flow cytometry. Flow cytometry profiles corresponding to 10 hr at 25° (rereplication positive control) and to 5 hr at 36° were compared (shaded background profiles). DNA content is indicated at the bottom.