TABLE 2.
Mutants isolated by EMS mutagenesis
| Method | No. of colonies screened |
No. picked |
No. characterized by respiration | Mutations isolated |
|---|---|---|---|---|
| 1 | 130,000 | 1583 | 146 | E1, E2, E3, E4, E7, E15 |
| 2 | 1.8 × 109, 7500a | 116 | 42 | E5, E6, E8, E9, E10, E11, E12, E13, E14 |
a
The first number indicates the total number of conidia treated with EMS and inoculated into antimycin A-containing liquid medium. The second number indicates the number of colonies that grew when the conidia remaining after filtration enrichment were plated.