Figure 1.—

DNA damage sensitivity of ccr4Δ and caf1Δ mutants and genetic interaction with dun1Δ. (A) Cells were grown to log phase and 10-fold serial dilutions starting from A₆₀₀ = 0.5 were spotted on YPD plates with or without HU or MMS. (B) Time course survival assays of the indicated strains in 100 mm HU. Aliquots were taken immediately before and every 4 hr after addition of HU and plated on YPD plates. Viable colonies were counted after 3 days of growth and survival was determined on the basis of numbers of colonies before HU addition. Error bars represent the standard deviation. wt, wild type.