TABLE 2.
Phylogenetic groups of α/β-hydrolase fold enzymes with a nucleophilic aspartate
| Groupa | Source(s) | Activity (putative) | No. of members (known, new) | Example(s) of members with known activity | Charge relay acid(s) (positionb) (frequency [%]) | Avg size (amino acids) |
|---|---|---|---|---|---|---|
| 1 | Bacterial, fungal | Epoxide hydrolase | 22 (1, 0) | BD8876 (49) | D (b) (100) | 300 |
| 1A | Bacterial | Epoxide hydrolase | 12 (0, 0) | D (b) (100) | 344 | |
| 2 | Bacterial, archaeal | Epoxide hydrolase | 26 (0, 3) | Bsueh, Draeh, Npueh 1 | D (b) (100) | 295 |
| Nematode | 4 (0, 0) | 367 | ||||
| 3 | Fungal | Epoxide hydrolase | 11 (0, 0) | D (b) (100) | 342 | |
| Bacterial | Epoxide hydrolase | 12 (0, 0) | 313 | |||
| Epoxide hydrolase-SDR | 12 (0, 0) | 591 | ||||
| 4 | Bacterial | Epoxide hydrolase | 27 (0, 1) | Npueh2, Ppueh | D (b) (100) | 308 |
| Fungal | Epoxide hydrolase | 6 (0, 0) | 295 | |||
| Bacterial | Haloalkane dehalogenase | 8 (5, 0) | Xanthobacter autotrophicus DhlA (23) | D (b) (50), E (a) (50) | 299 | |
| 5 | Animal | Epoxide hydrolase | 19 (13, 0) | Human micosomal EH (22), Manduca sexta juvenile hormone EH (18) | D (b) (25), E (b) (75) | 459 |
| Fungal | 22 (6, 0) | A. niger EH (3), R. glutinis EH (45) | 418 | |||
| Bacterial | 44 (4, 1) | BD9883 (49), Bfueh1 | 399 | |||
| 6 | Bacterial, fungal | Epoxide hydrolase | 14 (2, 0) | Corynebacterium sp. strain C12 EH (29), BD8877 (49) | D (a) (64), E (a) (36) | 293 |
| Bacterial | Haloacetate dehalogenase | 19 (1, 0) | Moraxella sp. DehL (24) | D (a) (100) | 297 | |
| 7 | Bacterial | Epoxide hydrolase | 4 (2, 0) | Agrobacterium radiobacter AD1 EH (35), BD10721 (49) | D (b) (100) | 292 |
| 8 | Animal | Epoxide hydrolase | 4 (3, 0) | Human soluble EH (10) | D (b) (100) | 549 |
| Plant | 15 (11, 0) | Potato EH (40) | 337 | |||
| Bacterial | 26 (0, 1) | Rpaeh2 | 329 |