TABLE 3.

Properties of MalE-EH fusion proteins

Enzyme	Origin	Groupg	Length (amino acids)	Molecular mass (kDa)a	Putative active-site residue				Expression level (% of total protein)	pNSO (compound 5) parameters		pNPGE (compound 10) parameters
					Nucleophile	Histidine	Acid	Tyrosine(s)		Activity (U mg−1)	Ed,f	Activity (U mg−1)	Ed,f
AraEchAb	Agrobacterium radiobacter AD1	7	294	34.1	D107	H275	D246	Y152, Y215	41	1.0	56 (R)	4.0	3.4 (S)
Bsuehc	Bacillus subtilis	2	286	32.8	D103	H265	D237	Y150, Y210	17	<0.002e	—	0.15	22 (R)
Bfueh1b	Burkholderia fungorum LB400	5	396	44.2	D182	H360	E333	Y239, Y308	29	0.16	45 (R)	0.80	5.3 (R)
Corehc	Corynebacterium sp. strain C12	6	286	32.1	D99	H264	D123	Y167, Y209	19	0.67	24 (R)	<0.002	—
Draehc	Deinococcus radiodurans R1	2	278	31.4	D104	H259	D231	Y150, Y205	36	0.11	21 (S)	0.75	1.8 (R)
MtuEphFb	Mycobacterium tuberculosis H37v		300	33.8	D108	H274	D246	Y153, Y213	13	<0.002	—	<0.002	—
Npuch1c	Nostoc punctiforme	2	289	33.4	D104	H267	D239	Y150, Y211	55	1.3	20 (S)	1.3	16 (R)
Npueh2b	Nostoc punctiforme	4	300	34.7	D112	H273	D246	Y154, Y217	39	<0.002	—	0.003	—
Ppuehb	Pseudomonas putida KT2440	4	299	34.3	D107	H267	D239	Y213	12	<0.002	—	<0.002	—
Rpach2b	Rhodopseudomonas palustris CGA009	8	316	34.7	D100	H294	D261	Y148, Y231	49	1.1	2.6 (R)	36	2.2 (S)
Scoeh6b	Streptomyces coelicolor A3(2)	6	292	32.1	D103	H270	E127	Y158, Y215	57	<0.002	—	<0.002	—
Tfuehb	Thermobifida fusca	5	393	43.3	D183	H366	D341	Y245, Y316	11	<0.002	—	<0.002	—

^aMolecular masses are for the EH segment of the fusion protein.

^bInitial activities were determined spectrophotometrically at a 0.5 mM substrate concentration with CFEs.

^cInitial activities were determined spectrophotometrically with (partially) purified protein.

^dThe enantiomer that is preferentially hydrolyzed is indicated in parentheses.

^eActivity below the detection limit (0.002 U mg⁻¹).

^f—, Activity too low for determination of an accurate E value.

^gSee Fig. 3.