TABLE 4.
Substrate ranges of cloned bacterial epoxide hydrolases
| Enzyme | Activity toward indicated epoxide substrate numbera:
|
||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Aromatic substrates
|
Aliphatic substrates
|
Cyclic substrates
|
|||||||||||||||||||
| 1 | 2 | 4 | 5 | 6 | 7 | 8 | 10 | 11 | 12 | 13 | 14 | 16 | 17 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | |
| AraEchAc | ++ | ++ | ++ | ++ | − | − | − | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | + | − | − | + | − | − |
| Bsuehb | − | − | − | − | − | − | − | ++ | − | − | − | − | − | − | ++ | − | − | − | − | − | − |
| Bfuehlc | ++ | ++ | ++ | ++ | − | − | − | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | − | − | − | + | − | − |
| Corehb | ++ | ++ | ++ | ++ | ++ | − | − | − | + | + | + | ++ | + | ++ | ++ | − | − | − | ++ | + | − |
| Draehb | ++ | ++ | ++ | ++ | − | + | ++ | ++ | ++ | ++ | − | ++ | ++ | ++ | ++ | − | − | − | − | − | − |
| MtuEphFc | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − |
| Npueh1b | ++ | ++ | ++ | ++ | − | + | − | ++ | ++ | ++ | − | ++ | ++ | ++ | ++ | − | − | − | + | − | − |
| Npueh2c | − | − | − | − | − | − | − | + | − | − | − | − | − | − | − | − | − | − | − | − | − |
| Ppuehc | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − |
| Rpaeh2c | ++ | ++ | ++ | ++ | − | ++ | ++ | ++ | ++ | ++ | + | + | ++ | ++ | ++ | + | − | − | − | − | − |
| Scoeh6c | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − |
| Tfuehc | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − |
a
All assays were done with the adrenaline test (46), with the exception of that for epoxide 10, for which conversion was monitored spectroscopically. Activities were scored as follows: −, <3 nmol mg−1 min−1; +, >3 nmol mg−1 min−1; and ++, >10 nmol mg−1 min−1. Epoxide structures are shown in Fig. 2.
b
Measurements were performed with purified protein.
c
Measurements were performed with desalted cell extracts.