TABLE 5.

Activities and enantioselectivities of novel group 2 epoxide hydrolases for terminal epoxides

Enzyme and parameter	Activity (μmol min−1 mg−1) or enantioselectivitya with indicated epoxide substrate numberc:
	Aromatic substrates							Aliphatic substrates
	1	2	3	4	5	9	10	11	15	16	17	18	19
Bsueh
Activity	<0.003	<0.003	<0.003	<0.003	<0.003	0.01	0.15	<0.003	<0.003	<0.003	<0.003	0.11	0.91
Enantioselectivity	—b	—	—	—	—	—	22 (R)	—	—	—	—	1.2 (R)	2.5 (R)
Draeh
Activity	0.42	0.06	0.68	0.19	0.11	0.85	0.75	0.07	0.04	0.31	4.3	13.8	25.6
Enantioselectivity	4.2 (S)	5.2 (S)	2.3 (S)	6.4 (S)	21 (S)	1.4 (R)	1.9 (R)	1.3 (S)	2.9 (R)	3.5 (R)	6.5 (R)	4.6 (R)	7.0 (R)
Npueh1
Activity	0.37	0.42	2.1	0.74	1.3	1.3	1.3	0.21	0.33	1.2	1.2	1.7	2.4
Enantioselectivity	2.3 (S)	1.9 (S)	4.6 (S)	1	17 (S)	3.7 (R)	16 (R)	1.3 (R)	1.5 (R)	1.2 (R)	1.7 (R)	1.7 (R)	1.8 (R)

^aThe preferentially hydrolyzed enantiomer is indicated in parentheses.

^b—, activity was too low to determine an accurate E value.

^cSee Fig. 2 for epoxide structures.