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. 2006 Apr;72(4):2905–2917. doi: 10.1128/AEM.72.4.2905-2917.2006

TABLE 7.

Activities of different fusion proteins for meso-epoxides

Enzyme Parameter value for indicated substrate
cis-2,3-Epoxybutane (substrate 20)
Cyclohexene oxide (substrate 23)
Activity (μmol min−1 mg−1) eep (%) E valued Activity (μmol min−1 mg−1) eep (%) E valued
AraEchAa 0.003 85 (2R,3R) 12 0.005 >99 (R,R) >200
Bfueh1a <0.002 c 0.035 89 (S,S) 17
Corehb <0.002 1.4 30 (R,R) 1.9
Npueh1b <0.002 0.016 97 (S,S) 66
Rpaeh2a 0.019 58 (2R,3R) 3.8 <0.002
a

Initial activities were determined at a 2 mM substrate concentration with cell extracts.

b

Activities were measured with (partially) purified protein.

c

—, activity was too low to determine the enantiomeric excess of the product (eep).

d

The E value was calculated from the eep according to equation 1.