TABLE 7.
Enzyme | Parameter value for indicated substrate
|
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---|---|---|---|---|---|---|
cis-2,3-Epoxybutane (substrate 20)
|
Cyclohexene oxide (substrate 23)
|
|||||
Activity (μmol min−1 mg−1) | eep (%) | E valued | Activity (μmol min−1 mg−1) | eep (%) | E valued | |
AraEchAa | 0.003 | 85 (2R,3R) | 12 | 0.005 | >99 (R,R) | >200 |
Bfueh1a | <0.002 | —c | — | 0.035 | 89 (S,S) | 17 |
Corehb | <0.002 | — | — | 1.4 | 30 (R,R) | 1.9 |
Npueh1b | <0.002 | — | — | 0.016 | 97 (S,S) | 66 |
Rpaeh2a | 0.019 | 58 (2R,3R) | 3.8 | <0.002 | — | — |
Initial activities were determined at a 2 mM substrate concentration with cell extracts.
Activities were measured with (partially) purified protein.
—, activity was too low to determine the enantiomeric excess of the product (eep).
The E value was calculated from the eep according to equation 1.