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. 2006 Apr;72(4):2849–2855. doi: 10.1128/AEM.72.4.2849-2855.2006

FIG. 4.

FIG. 4.

Epifluorescent micrographs of V. cholerae O1 detected in environmental samples collected in December 2004. Samples were stained with direct fluorescent monoclonal antibody (DFA) specific for V. cholerae serovar O1 (obtained from New Horizon Diagnostic) and visualized (18, 19) by using an epifluorescence microscope (Olympus model BX51, BX2 series). Microscopic images were captured digitally (Olympus DP20) and processed by using Adobe Photoshop version 5. (A and B) V. cholerae O1 in the initial stage of in situ biofilm formation, either free-swimming (A) or attached to plankton or other aggregates (B) in samples collected from pond site 1; (C and D) V. cholerae O1 in the initial stage of in situ biofilm formation in a sample collected from ponds at sites 5 and 6, respectively.