TABLE 2.
Detection and isolation of V. cholerae O1, O139, and non-O1/non-O139 from water and two size fraction plankton samples collected from six pond sites in Mathbaria, adjacent to the Bay of Bengal
| Site | Samplea | No. of samplesb | No. of samples positive
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Culturec
|
DFA
|
Multiplex-PCR
|
||||||||
| O1 | O139 | Non-O1/O139 | O1 | O139 | O1 rfb | O139 rfb | ctxA | |||
| 1 | Water | 10 | 0 | 0 | 6 | 6 | 0 | 0 | 0 | 1 |
| 20-μm fraction (plankton) | 10 | 1 | 0 | 0 | 5 | 1 | 0 | 1 | 1 | |
| 64-μm fraction (plankton) | 10 | 1 | 0 | 4 | 4 | 0 | 0 | 0 | 3 | |
| 2 | Water | 17 | 0 | 0 | 10 | 7 | 2 | 0 | 0 | 0 |
| 20-μm fraction (plankton) | 17 | 0 | 0 | 10 | 3 | 3 | 1 | 1 | 3 | |
| 64-μm fraction (plankton) | 17 | 0 | 0 | 7 | 5 | 5 | 0 | 1 | 0 | |
| 3 | Water | 18 | 0 | 1 | 8 | 5 | 4 | 0 | 2 | 3 |
| 20-μm fraction (plankton) | 18 | 0 | 2 | 9 | 8 | 3 | 1 | 3 | 2 | |
| 64-μm fraction (plankton) | 18 | 0 | 0 | 11 | 5 | 3 | 0 | 3 | 3 | |
| 4 | Water | 18 | 0 | 0 | 8 | 4 | 1 | 0 | 1 | 0 |
| 20-μm fraction (plankton) | 18 | 0 | 0 | 10 | 3 | 4 | 0 | 0 | 1 | |
| 64-μm fraction (plankton) | 18 | 0 | 0 | 9 | 4 | 5 | 0 | 0 | 2 | |
| 5 | Water | 18 | 1 | 0 | 5 | 2 | 1 | 2 | 2 | 4 |
| 20-μm fraction (plankton) | 18 | 1 | 0 | 6 | 6 | 2 | 1 | 1 | 1 | |
| 64-μm fraction (plankton) | 18 | 1 | 0 | 7 | 3 | 2 | 1 | 0 | 4 | |
| 6 | Water | 18 | 1 | 0 | 4 | 3 | 1 | 1 | 2 | 3 |
| 20-μm fraction (plankton) | 18 | 1 | 0 | 6 | 6 | 5 | 1 | 1 | 3 | |
| 64-μm fraction (plankton) | 18 | 1 | 1 | 5 | 3 | 2 | 0 | 0 | 0 | |
a
Six each of water and two size fractions of plankton samples were collected per round every fortnight.
b
Sites 1 and 2 could not be sampled for all 18 rounds.
c
One representative colony from each culture-positive sample was isolated and stored for further study.