TABLE 2.
Function class | Mutant namea | Gene | STM no.b | Insertion site/no. of bpc | Description | Survival ratiof
|
|
---|---|---|---|---|---|---|---|
SSCd | ATRe | ||||||
Protein fate | SB 110/1F1 | dnaK | STM0012 | −127/1,917 | Chaperone | 0.032 | 0.10 |
Protein fate | SB 156/18G1 | STM2691 | +275/2,181 | Putative ABC transmembrane transporter | 0.37 | 0.44 | |
Amino acid biosynthesis | SB 200/31G4 | usg | STM2369 | +175/1,014 | Putative aspartate-semialdehyde dehydrogenase | 0.25 | 0.10 |
Regulatory functions | SB 213/24F5 | barA | STM2958 | +313/2,757 | Sensor kinase for virulence gene expression | 0.050 | 1.13 |
Energy metabolism | SB 135/16H3 | STM1382 | +616/1,227 | Putative regulatory protein | 0.066 | 0.27 | |
Central intermediary metabolism | SB 104/2G3 | dgt | STM0208 | +780/1,518 | dGTP triphosphohydrolase | 0.00052 | 1.30 |
Cellular processes | SB 117/8B6 | sopB/sigD | STM1091 | +709/1,686 | Inositol phosphate phosphatase | 0.015 | 0.35 |
Cellular processes | SB 162/18F6 | traL | PSLT078 | +218/312 | Conjugative transfer | 0.017 | 0.20 |
Unclassified | SB 157/18D2 | STM2585 | +468/546 | Transposase-like protein | 0.086 | 0.50 | |
Unclassified | SB 115/8G3 | pefA | PSLT018 | +108/519 | Plasmid-encoded fimbriae | 0.61 | 1.13 |
Unclassified | SB 114/6C6 | pefC | pSLT017 | +175/2,409 | Plasmid-encoded fimbriae | 0.031 | 0.27 |
Unclassified | SB 116/8E6 | rfaL | STM3713 | +211/1,215 | O-antigen ligase | 0.45 | 0.92 |
Cell envelope | SB 113/7B2 | asmA | STM2120 | +718/1,866 | Outer membrane protein assembly | 0.0035 | 1.11 |
Cell envelope | SB 126/10B1 | rfbM/manC | STM2084 | +291/1,440 | Mannose-1-phosphate guanylyltransferase | 2.94 | 57.73 |
Cell envelope | SB 105/11B1 | ynaI | STM1663 | +430/1,032 | Putative integral membrane protein | 0.023 | 48.67 |
Cell envelope | SB 209/35C6 | ynaI | STM1663 | +94/1,032 | Putative integral membrane protein | 0.012 | 84.25 |
Transcription | SB 130/12B6 | pnp | STM3282 | +1,584/2,136 | Polynucleotide phosphorylase | 0.00062 | 0.39 |
Mobile and extrachromosomal element functions | SB 166/18E4 | mig-3 | STM1868 | +532/882 | Macrophage-inducible protein | 0.11 | 0.86 |
Unknown function | SB 134/16H1 | yjeA/poxR | STM4344 | +713/978 | Lysyl-tRNA synthetase | 0.053 | 0.06 |
Strain name/STM pool.
The STM and pSLT prefixes indicate the gene numbers on the chromosome and virulence plasmid, respectively, as assigned by the S. enterica serovar Typhimurium LT2 complete genome sequencing project.
Location of transposon insertion site/number of base pairs within the gene.
Following 1 h of pH 4.4 adaptation, a 10-min challenge of each mutant was performed independently in the SSC assay.
Following 1 h of pH 4.4 adaptation, a 90-min challenge of each mutant was performed independently in the ATR assay at pH 3.0.
Data are presented as the ratio of the percent survival of the mutant divided by the percent survival of the wild-type strain; therefore, mutants with a ratio below 1 did not survive as well as the wild-type strain, and mutants with a ratio above 1 survived better than the wild-type strain.