. 2006 Apr;72(4):2829–2836. doi: 10.1128/AEM.72.4.2829-2836.2006

TABLE 2.

Characterization of signature-tagged mutants

Function class	Mutant name^a	Gene	STM no.^b	Insertion site/no. of bp^c	Description	Survival ratio^f
Function class	Mutant name^a	Gene	STM no.^b	Insertion site/no. of bp^c	Description	SSC^d	ATR^e
Protein fate	SB 110/1F1	dnaK	STM0012	−127/1,917	Chaperone	0.032	0.10
Protein fate	SB 156/18G1		STM2691	+275/2,181	Putative ABC transmembrane transporter	0.37	0.44
Amino acid biosynthesis	SB 200/31G4	usg	STM2369	+175/1,014	Putative aspartate-semialdehyde dehydrogenase	0.25	0.10
Regulatory functions	SB 213/24F5	barA	STM2958	+313/2,757	Sensor kinase for virulence gene expression	0.050	1.13
Energy metabolism	SB 135/16H3		STM1382	+616/1,227	Putative regulatory protein	0.066	0.27
Central intermediary metabolism	SB 104/2G3	dgt	STM0208	+780/1,518	dGTP triphosphohydrolase	0.00052	1.30
Cellular processes	SB 117/8B6	sopB/sigD	STM1091	+709/1,686	Inositol phosphate phosphatase	0.015	0.35
Cellular processes	SB 162/18F6	traL	PSLT078	+218/312	Conjugative transfer	0.017	0.20
Unclassified	SB 157/18D2		STM2585	+468/546	Transposase-like protein	0.086	0.50
Unclassified	SB 115/8G3	pefA	PSLT018	+108/519	Plasmid-encoded fimbriae	0.61	1.13
Unclassified	SB 114/6C6	pefC	pSLT017	+175/2,409	Plasmid-encoded fimbriae	0.031	0.27
Unclassified	SB 116/8E6	rfaL	STM3713	+211/1,215	O-antigen ligase	0.45	0.92
Cell envelope	SB 113/7B2	asmA	STM2120	+718/1,866	Outer membrane protein assembly	0.0035	1.11
Cell envelope	SB 126/10B1	rfbM/manC	STM2084	+291/1,440	Mannose-1-phosphate guanylyltransferase	2.94	57.73
Cell envelope	SB 105/11B1	ynaI	STM1663	+430/1,032	Putative integral membrane protein	0.023	48.67
Cell envelope	SB 209/35C6	ynaI	STM1663	+94/1,032	Putative integral membrane protein	0.012	84.25
Transcription	SB 130/12B6	pnp	STM3282	+1,584/2,136	Polynucleotide phosphorylase	0.00062	0.39
Mobile and extrachromosomal element functions	SB 166/18E4	mig-3	STM1868	+532/882	Macrophage-inducible protein	0.11	0.86
Unknown function	SB 134/16H1	yjeA/poxR	STM4344	+713/978	Lysyl-tRNA synthetase	0.053	0.06

Strain name/STM pool.

The STM and pSLT prefixes indicate the gene numbers on the chromosome and virulence plasmid, respectively, as assigned by the S. enterica serovar Typhimurium LT2 complete genome sequencing project.

Location of transposon insertion site/number of base pairs within the gene.

Following 1 h of pH 4.4 adaptation, a 10-min challenge of each mutant was performed independently in the SSC assay.

Following 1 h of pH 4.4 adaptation, a 90-min challenge of each mutant was performed independently in the ATR assay at pH 3.0.

Data are presented as the ratio of the percent survival of the mutant divided by the percent survival of the wild-type strain; therefore, mutants with a ratio below 1 did not survive as well as the wild-type strain, and mutants with a ratio above 1 survived better than the wild-type strain.