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(A) Heterologous expression of NysL in Escherichia coli as a His-tagged protein. Lanes: 1, crude cell extract; 2, flowthrough; 3, wash; 4, eluted His-NysL; 5, molecular weight marker. (B) Enzymatic assay monitored by HPLC and LC-MS showing conversion of 10-deoxynystatin to nystatin by His-NysL and the redox partners.
