TABLE 2.
Purification step | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Yield (%) | Purification (fold) |
---|---|---|---|---|---|
Crude extract | 1,332 | 373 | 0.28 | 100 | 1 |
Mono Q | 117 | 214 | 1.83 | 57 | 6.5 |
Superose 6 | 62 | 156 | 2.52 | 42 | 9 |
Sephacryl S200 | 19 | 75 | 3.94 | 20 | 14 |