TABLE 3.
Summary of the kinetic parameters of CpdB and its mutants
| Substrate | Km (μM) | kcat (s−1) | kcat/Km (M−1 s−1) |
|---|---|---|---|
| Cyclododecanone (C12) | |||
| CpdB (wt)a | 34.0 ± 1.2 | 2.7 | 7.9 × 104 |
| CpdB (S261A) | 24.5 ± 2.5 | 3.1 | 1.3 × 105 |
| CpdB (G242A) | 38.3 ± 2.3 | 2.0 | 5.2 × 104 |
| Cyclotridecanone (C13) | |||
| CpdB (wt) | 12.5 ± 0.8 | 6.5 | 5.2 × 105 |
| CpdB (S261A) | 9.1 ± 1.5 | 5.3 | 5.8 × 105 |
| CpdB (G242A) | 14.4 ± 0.8 | 3.6 | 5.2 × 105 |
| Cyclopentadecanone (C15) | |||
| CpdB (wt) | 5.8 ± 0.9 | 4.2 | 7.2 × 105 |
| CpdB (S261A) | 7.0 ± 2.1 | 4.9 | 7.0 × 105 |
| CpdB (G242A) | 6.5 ± 2.1 | 2.7 | 4.2 × 105 |
| NADPH | |||
| CpdB (wt) | 24.0 ± 2.1 | NAb | NA |
| CpdB (S261A) | 7.8 ± 1.2 | NA | NA |
| CpdB (G242A) | 32.4 ± 3.0 | NA | NA |
a
wt, wild type.
b
NA, not applicable.