TABLE 2.

Primers used in this work

Primer	Sequence (5′→3′)a	Insert
PromBCY1-preFRT	5′-CCATTACACGCATTTCACGCATACATAAATAGGAAGATCATTTACAACA AGCAGATTATTTTCAAAAGACAACAGTAAGAATAAACGATGTATCACG AGGCCCTTTCG-3′	KAN-FLP-FRT
TermBCY1-postFRT	5′-GAAGGAAGAAGAAAGAAAAAGGGAACAGAAGAAGAAGAAAAAGAGA AAGGAAATTCATGTGGATTTAAGATCGCTTCCCCTTTTTACTTAGATAA GCTGTCAAACATGAG-3′	KAN-FLP-FRT
PreFRT′-iBCY3′	5′-CGAAAGGGCCTCGTGATAGGGCAAAGAAGATACCATCG-3′	iBCY1
5′GEM-iBCY5′	5′-CGACTCACTATAGGGCGAATCTAGAAGAGGAGCATACGACTTCG-3′	iBCY1
PostFRT′-tBCY5′	5′-CTCATGTTTGACAGCTTATCTTCTTCCTTCAACGTCTACG-3′	tBCY1
3′GEM-tBCY3′	5′-GGTGACACTATAGAATACTCTAGAGCTGTCTTGTAGATCCTTTGG-3′	tBCY1
PreFRT′-StopsBCY3′	5′-CGAAAGGGCCTCGTGATATTATCATGCAGGACCCAGTAAACGTTG-3′	sBCY1
5′GEM-sBCY5′	5′-CGACTCACTATAGGGCGAATCTAGAGGACTACTTCTATGTCGTCG-3′	sBCY1

^apHFKH plasmid hybridization sites are underlined. pGPCR3 plasmid hybridization sites are double underlined. Italics indicate nucleotides added to create an XbaI restriction site. Boldface type indicates added stop codons.