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. 2006 Apr;72(4):2564–2572. doi: 10.1128/AEM.72.4.2564-2572.2006

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FIG. 1. — Topographical images of colonies of E. coli O157:H7 strains 43895OW (saw) (A) and 43895OR (rdar) (B) grown and fixed on YESCA agar for 48 h at 28°C. (C) Negatively stained TEM of dispersed colonies of strain 3895OR (rdar) harvested from a YESCA plate grown for 48 h at 28°C and stained with uranyl acetate. (D) Immunoblot of formic acid-treated cells collected from YESCA plates after 48 h at 28°C. Separated proteins of strains 43895OR (lane 1) and 43895OW (lane 2) reacted with anti-CsgA antibody. The arrow indicates the position of the 15-kDa marker. The predicted monomeric form of CsgA is 15.1 kDa.