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. 2006 Apr;72(4):2414–2420. doi: 10.1128/AEM.72.4.2414-2420.2006

TABLE 1.

Oligonucleotide primer pairs employed in RT-PCR assaysa

Primer name Primer sequence (5′-3′) Primer position on the genome Length of amplified product
ABPV 23f GTG CTA TCT TGG AAT ACT AC 7928-7947 618
ABPV 24r AAG GYT TAG GTT CTA CTA CT 8527-8546
BQCV 3f AGT AGT TGC GAT GTA CTT CC 252-277 472
BQCV 4r CTT AGT CTT ACT CGC CAC TT 710-729
CBPV 111f TGT CGA ACT GAG GAT CTT AC 111-130 315
CBPV 426r GAC CTG ATT AAC GAC GTT AG 407-426
DWV 2345f ATT GTG CCA GAT TGG ACT AC 2345-2364 434
DWV 2779r AGA TGC AAT GGA GGA TAC AG 2760-2997
KBV 5406f GAT GAA CGT CGA CCT ATT GA 5406-5425 395
KBV 5800r TGT GGG TTG GCT ATG AGT CA 5781-5800
SBV 1f ACC AAC CGA TTC CTC AGT AG 221-240 487
SBV 2r CCT TGG AAC TCT GCT GTG TA 689-708
a

Nucleotide positions refer to the published sequences of ABPV (GenBank accession number AF150629), BQCV (accession number AF125252), CBPV (accession number AF375659), DWV (accession number AJ489744), KBV (accession number AY275710), and SBV (accession number AF092924). f, forward primer; r, reverse primer.