FIG. 2.

Chemiluminograms showing hybridization patterns of digoxigenin-labeled amplicons obtained after PCR amplification of DNAs of pure cultures of P. aristosporium (A) and P. grandisporangium (B). Amino-linked oligonucleotides were spotted in duplicate, hybridized overnight, exposed on Kodak X-ray film for 45 min, and developed as described in Materials and Methods. Pairs of diagonally arranged dark spots indicate positive hybridization signals. Spots A1 to A5 are universal, family- or genus-specific oligonucleotides as described in Table 1, while A20 is a validating standard for digoxigenin efficiency. Chemiluminograms were repeated at least once, and similar patterns were obtained.