FIG. 3.

Hybridization patterns of digoxigenin-labeled PCR amplicons obtained by direct processing of soil. One representative sample out of four replicates that had very similar hybridization patterns and showed the same species complex. Spots A1 to A5 are universal, family- or genus-specific oligonucleotides as described in Table 1. Chemiluminograms were repeated at least twice, and similar patterns were obtained. The species detected in all soil samples were P. acanthicum (G12, H12), P. arrhenomanes (B16, C16, A16), P. volutum (G18, I18), P. torulosum (A18, C18, D18), P. vanterpolii (H19), P. monospernum (B13, D13), P. acrogynum (C7), P. minus (F7), P. attrantheridium (F9), P. sylvaticum (C10, D10, E10), P. heterothallicum (D5), P. nunn (G6, H6), and P. rostratum (I20).