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Use of receiver operating curves to determine quantitative Legionella 16S rRNA gene PCR (A) and mip PCR (B) cutoff values (GU/liter) for identification of samples containing ≥103 CFU/liter legionellae among hot water system samples analyzed at Lyon (study 1) and Grenoble (study 2). For mip PCR (B), samples yielding non-L. pneumophila legionellae by culture (six samples in study 1 and one sample in study 2) were excluded. Se, sensitivity; Sp, specificity.
