TABLE 1.
Primer sequences, restriction enzymes, fragment sizes, and parental genotypes for eight sequence-characterized amplified region markers
| Locus | Primer sequence (5′-3′)a | Restriction enzyme | Allele | Characteristic DNA fragment (bp)b | Genotypes
|
|
|---|---|---|---|---|---|---|
| S608-2 | C9 | |||||
| PR3 | CGCCACATGTTTCCCTTCAAT | HinfI | 1 | 630, 280 | 1 | 2/2 |
| TGGAGCATCATCAGGACTTGG | 2 | 400, 210 | ||||
| PR21 | GTCAAGAGCGTTAATCAA | HinfI | 1 | 270, 110 | 1 | 2/2 |
| ACGTCGGTGAAATCCATT | 2 | 380 | ||||
| PR28 | GAACCAAATTTACTGTGA | HinfI | 1 | 750, 540 | 1 | 4/4 |
| GAGAAGCAACAAAATATG | 4 | 1,100, 300 | ||||
| PR46 | CAGGCAGTTCAAAAGCCAGT | HinfI | 1 | 330, 240 | 1 | 3/3 |
| ACAATTTCCGAGATCACCGA | 3 | 480, 240 | ||||
| PR4 | TTGCCGCTTCACTCGCCGTT | HinfI | 1 | 230, 210 | 1 | 1/2 |
| CCGTTGGTGAACCAGCGGAGGGATC | 2 | 340, 230, 210 | ||||
| PR6 | CAATCTCAAGCTTGCCTGG | None | 1 | 830 | 1 | 1/2 |
| AGGTGACATGTCAGAAGCGC | 2 | 1,250 | ||||
| PR30 | GACTAACCGTCGGAAT | RsaI | 1 | 400, 160 | 1 | 1/2 |
| TCACACAGGAAACAGC | 2 | 260, 160 | ||||
| PR50 | CCATCTCTCGTGATGAGCTTG | HpaII | 1 | 730, 250 | 1 | 1/3 |
| TAAGCAAAAGCGACAGCGAT | 3 | 900, 250 | ||||