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. 2006 Apr;72(4):2366–2372. doi: 10.1128/AEM.72.4.2366-2372.2006

TABLE 1.

Primer sequences, restriction enzymes, fragment sizes, and parental genotypes for eight sequence-characterized amplified region markers

Locus Primer sequence (5′-3′)a Restriction enzyme Allele Characteristic DNA fragment (bp)b Genotypes
S608-2 C9
PR3 CGCCACATGTTTCCCTTCAAT HinfI 1 630, 280 1 2/2
TGGAGCATCATCAGGACTTGG 2 400, 210
PR21 GTCAAGAGCGTTAATCAA HinfI 1 270, 110 1 2/2
ACGTCGGTGAAATCCATT 2 380
PR28 GAACCAAATTTACTGTGA HinfI 1 750, 540 1 4/4
GAGAAGCAACAAAATATG 4 1,100, 300
PR46 CAGGCAGTTCAAAAGCCAGT HinfI 1 330, 240 1 3/3
ACAATTTCCGAGATCACCGA 3 480, 240
PR4 TTGCCGCTTCACTCGCCGTT HinfI 1 230, 210 1 1/2
CCGTTGGTGAACCAGCGGAGGGATC 2 340, 230, 210
PR6 CAATCTCAAGCTTGCCTGG None 1 830 1 1/2
AGGTGACATGTCAGAAGCGC 2 1,250
PR30 GACTAACCGTCGGAAT RsaI 1 400, 160 1 1/2
TCACACAGGAAACAGC 2 260, 160
PR50 CCATCTCTCGTGATGAGCTTG HpaII 1 730, 250 1 1/3
TAAGCAAAAGCGACAGCGAT 3 900, 250
a

Primers for PR3, PR4, PR6, and PR21 have been described previously (2, 6, 8, 19).

b

Approximate sizes.