FIG. 4.

PFGE analysis of the “Candidatus Arsenophonus arthropodicus” genome following removal of extrachromosomal elements. Restriction fragments and markers were resolved under three distinct sets of electrophoresis conditions, as indicated below each gel photograph. The lanes are labeled as follows: H, high-range PFGE marker (New England BioLabs), a concatemer of a 48.5-kbp λ DNA fragment; L, low-range PFGE marker (New England BioLabs), a HindIII digest of λ DNA mixed with 48.5-kbp λ DNA concatemers (asterisk marks the 48.5-kbp λ DNA fragment); N and A, NotI and AscI digests, respectively, of chromosomal DNA isolated from “Candidatus Arsenophonus arthropodicus.” The smallest λ concatemer visible in lane H on the gel from the 36-h run is the 145.5-kbp fragment. The sizes of restriction fragments from the NotI and AscI digests are presented in the adjacent table. The total sizes of the NotI and AscI fragments were estimated to be 3,457 kbp and 3,561 kbp, respectively.