FIG. 2.

Detection of E. sakazakii by PCR amplification of the ompA gene. Primer pair ESSF and ESSR and the amplification conditions and gel electrophoresis method described in Materials and Methods were employed. Lanes: M, molecular weight marker (GeneRuler DNA Ladder Mix; Fermentas, Hanover, Md.); 1 to 17, PCR products from E. sakazakii strains 1 to 17, as listed in Table 1; 18 to 68, PCR products from negative control DNA from bacterial strains 18 to 68, as listed in Table 1; C, control PCR run without any template DNA.