Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Sensitivity of the PCR for detection of E. sakazakii in pure cultures. Primer pair ESSF and ESSR and the template preparation, amplification conditions, and gel electrophoresis method described in Materials and Methods were employed. Lanes: M, molecular weight marker (GeneRuler DNA Ladder Mix; Fermentas, Hanover, Md.); 1 to 9, PCR products amplified from samples containing 108 to 100 CFU/ml of E. sakazakii (ATCC 51329); 10, control PCR run without any template DNA.
