FIG. 5.
Detection of E. sakazakii in reconstituted infant formula by PCR after an enrichment step of 8 h. Primer pair ESSF and ESSR and the template preparation, amplification conditions, and gel electrophoresis method described in Materials and Methods were employed. Lanes: M, molecular weight marker (GeneRuler DNA Ladder Mix; Fermentas, Hanover, Md.); 1 to 6, PCR products amplified from infant formula containing 103 to 10−2 CFU/ml of E. sakazakii (ATCC 51329) after 8 h of enrichment; 7, control PCR run with uninoculated, reconstituted infant formula after 8 h of enrichment.