TABLE 1.

Bacterial strains and plasmids used directly in this study

Strain or plasmid	Description	Source or reference
Strains
E. coli
DH1	F−supE44 hsdR17 recA1 gyrA96 relA1 endA1 thi-1 λ−	11
DH1M	DH1 msbB	This work
DH1R	DH1::rbpA	This work
B. subtilis
168	trpC2	13
168-mpr	trpC2 mpr	This work
168-nprE	trpC2 nprE	This work
Plasmids
pCR2.1-TOPO	Vector for cloning PCR products	Invitrogen
pKO3	Source of cat in pTOPO-DifCAT	17
pTOPO-DifCAT	Precursor E. coli Xer-cise plasmid	This work
pQR163	Source of rbpA	27
prbpA-DifCAT	Precursor rbpA integration Xer-cise plasmid	This work
pTP223	Lambda Red helper plasmid	18
pypmP::CAT	Source of cat in pTOPO-bac.DifCAT	G. Homuth
pTOPO-bac.DifCAT	Precursor B. subtilis Xer-cise plasmid	This work
pmpr-DifCAT	mpr deletion Xer-cise plasmid	This work
pnprE-DifCAT	nprE deletion Xer-cise plasmid	This work