TABLE 1.
Bacterial strains and plasmids used directly in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH1 | F−supE44 hsdR17 recA1 gyrA96 relA1 endA1 thi-1 λ− | 11 |
| DH1M | DH1 msbB | This work |
| DH1R | DH1::rbpA | This work |
| B. subtilis | ||
| 168 | trpC2 | 13 |
| 168-mpr | trpC2 mpr | This work |
| 168-nprE | trpC2 nprE | This work |
| Plasmids | ||
| pCR2.1-TOPO | Vector for cloning PCR products | Invitrogen |
| pKO3 | Source of cat in pTOPO-DifCAT | 17 |
| pTOPO-DifCAT | Precursor E. coli Xer-cise plasmid | This work |
| pQR163 | Source of rbpA | 27 |
| prbpA-DifCAT | Precursor rbpA integration Xer-cise plasmid | This work |
| pTP223 | Lambda Red helper plasmid | 18 |
| pypmP::CAT | Source of cat in pTOPO-bac.DifCAT | G. Homuth |
| pTOPO-bac.DifCAT | Precursor B. subtilis Xer-cise plasmid | This work |
| pmpr-DifCAT | mpr deletion Xer-cise plasmid | This work |
| pnprE-DifCAT | nprE deletion Xer-cise plasmid | This work |