FIG. 6.

Fermentation run of A. nidulans R21OLAB7 (wild type) and three pkcA antisense RNA-encoding transformants (PkcAinv1.2, PkcAinv1.3, PkcAinv1.5) with lactose plus cyclopentanone or glucose as the carbon source. Data for each time point are means and standard deviations for three simultaneously harvested flasks (for dry weight, data represent only one flask). (A, B, and C) Glucose was used as the carbon source (repressing conditions). (D, E, and F) Lactose was used as the carbon source, and 10 mM cyclopentanone was added to the medium (inducing conditions). Results were expressed as dry weight (A and D), expression of the aatAp-lacZ fusion gene determined as β-Gal specific activity (B and E), or penicillin titer (C and F).