TABLE 1.
Fungal strains, bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and/or phenotype | Source or reference |
|---|---|---|
| Strains | ||
| Aspergillus nidulans | ||
| R21 | pabaA1 yA2 | 20 |
| LOGOAnBH1 | pyrG89 biA1 fwA1 bga0 argB2::pOLAB1 (aatAp-lacZ) pRG1 pLOGO-AnBH1 (anbH1-egfp) ArgB+ PyrG+ | 29 |
| OLAB1URA | pyrG89 biA1 fwA1 bga0 argB2::pOLAB1 (aatAp-lacZ) ArgB+ | 15 |
| AXB4A | bga0 biA1 argB2::pAXB4A (acvAp-uidA ipnAp-lacZ) ArgB+ | 11 |
| HapB-eGFP | pyrG89 pabaA1 fwA1 bga0 argB2::pAXB4A (acvAp-uidA ipnAp-lacZ) pHapB-GFP (hapB-egfp) ArgB+ PyrG+ | 44 |
| Palp32.3 | pyrG89 pabaA1 fwA1 bga0 argB2::pAXB4A (acvAp-uidA, ipnAp-lacZ) ΔsuAprgA1 paba1p32 ArgB+ PyrG+ PabaA+ | A. Gehrke, unpublished data |
| R21OLAB7 | pabaA1 yA2 argB2::pOLAB1 (aatAp-lacZ) ArgB+; progeny from a cross of strains R21 and OLAB1URA | This study |
| C2.3 | pyrG89 biA1 fwA1 bga0 argB2::pOLAB1 (aatAp-lacZ) pLOGO-AnBH1 (anbH1-egfp) ArgB+ | This study |
| PkcAinv1.1, -1.2, -1.3, -1.4, -1.5 | pyrG89 fwA1 bga0 biA1 argB2::pOLAB1 (aatAp-lacZ); pLOGO-AnBH1 (anbH1-egfp) (pAL4-pkcAinv)n ArgB+ PyrG+ | This study |
| Escherichia coli TOP10F′ | F′ [lacIq Tn10 (Tetr)] mcrA Δ(mrr-hsdRMS-mcrBC), φ80d/lacZΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen, The Netherlands |
| Bacillus calidolactis C953 | 11 | |
| Plasmids | ||
| pCR2.1TOPO | Ampr | Invitrogen, The Netherlands |
| pAL4 | Amprpyr-4 alcAp | 52 |
| pAL4-pkcAinv | Amprpyr-4 alcAp-pkcAantisense | This study |