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Cloning primers for heterologous expression
| Template | Oligonucleotide sequence (5′-3′)a | Restriction site |
|---|---|---|
| ite | AAGGTTCCATATGTTCACCACAAAATT AGCCGAAAAGGTC | NdeI |
| AAGGTTCAAGCTTTTACTACGGCTGCA CCGTCTTTATACC | HindIII | |
| iclB | AAGGTTCCATATGCGTGAACGCCTTAG | NdeI |
| AAGGTTCGGATCCTGCCTCCACCGACTC | BamHI | |
| iclS | AAGGTTCCATATGGAATCGAAAGTCAG | NdeI |
| AAGGTGGATCCGGCCGCTGCCAGG | BamHI |
Restriction sites are underlined.
