TABLE 3.

Sequences of N termini, tryptic peptides, and deduced primers

Peptide	Amino acid sequence (N-C)a	Deduced oligonucleotide sequence
IDS-epimerase
N terminus	MFTTKLAEKVVSAWKAKISQPALKAAQDb	AARGCNGCNCARGACGG (5′-3′)
Peptide 1	AAQDGVIDTVAAALGGVTEH
Peptide 2	WGVNQR
Peptide 3	F(S/X)IEANIGAAL(F/L)DGEV(S/X)LA(S/X)FEIE
Peptide 4	FDMPSETTFSGTTGY(Y/X)DIVVH	GTYTCNSWNGGCATRTCGAA (3′-5′)
BY6-lyase
N terminus	MRERLSASPNELIVKHLIGPRLFGNLDRDFLEM(X/S)KVNb	NGMGAYTTYYTNGAAATG (5′-3′)
Peptide 1	TGELLGFDGLIENSLDAVGSR	ATNARGCCRTCRAAGCC (3′-5′)
Peptide 2	NTIASVVIGNAPQLTSDE
Peptide 3	FNLEHALIEDVGAD
Peptide 4	TVMTGYTHGQPGQPI
SLRS7-lyase
N terminus	MESKVS(R/X)(X/R)LNEPLAK	GTSWSSCGSCGSCTSAACGAACC (5′-3′)
Peptide 1	QFAYLTEVNQAHL(L/X)MXX
Peptide 2	QVTADGLGFGSLVHN(T/X)LDA(X/V)X
Peptide 3	VAQDFYVWSTPEFGLL(X/S)F(X/P)Q(X/Q)XX
Peptide 4	(X/C)(V/L)(T/S)(L/L)(D/F)(E/F)(S/L)(V/V)(A/V)(T/G)
	(D/S) (V/L)(E/A) (D/P)(G/K)LVADAGLSF
Peptide 5	ASEFADAVMPGYTHLQPAQ(P/X)ITFG	ACGGSCCGATRTGSGTRGASGTCGG (3′-5′)

^aSegments used for the design of oligonucleotides for PCR are underlined.

^bFrom reference 9.