TABLE 1.
Summary of purification of MEHP hydrolasea
| Purification step | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Yield (%) | Purification (n-fold) |
|---|---|---|---|---|---|
| Cell extract | 1,540 | 473 | 0.31 | 100 | 1 |
| DE52 | 20.9 | 154 | 7.37 | 32.6 | 23.8 |
| TOYOPEARL | 11.3 | 101 | 8.94 | 21.4 | 28.8 |
| Superdex | 3.55 | 37.0 | 10.4 | 7.82 | 33.5 |
| Bio-Gel HTP | 0.980 | 16.7 | 17.0 | 3.53 | 54.8 |
a
One unit was defined as the amount of protein that converted 1 μmol of MEHP per min to phthalic acid. Five liters of culture was used for purification. The reactions were conducted at 45°C for 5 min. HPLC was used for analysis of the activity.